PENGARUH SUPLEMENTASI ISOFLAVON KEDELAI TERHADAP WANITA PENDERITA AKNE VULGARIS : Kajian Jumlah Lesi, Dihydrotestosterone, Toll-Like Receptor-2, dan Interleukin-8

Abstract

Latar belakang : Akne vulgaris (AV) merupakan penyakit inflamasi kulit yang paling sering dijumpai. Isoflavon kedelai telah terbukti sebagai antiandrogen dan antiinflamasi. Tujuan penelitian ini membuktikan pengaruh isoflavon kedelai terhadap lesi AV akibat penurunan kadar dihydrotestosterone (DHT), Toll-like receptor-2 (TLR-2), Interleukin-8 (IL-8) pada wanita penderita AV. Metoda : Randomized pre and post test control design. Penelitian pendahuluan dengan besar sampel 25 orang, dirandomisasi dalam kelompok plasebo, isoflavon 40 mg, 80 mg, 120 mg, 160 mg, lama penelitian 4 minggu. Penelitian lanjutan dengan 40 sampel, dirandomisasi dalam kelompok kontrol dan perlakuan, lama penelitian 12 minggu. Variabel bebas isoflavon kedelai, varibel terikat lesi AV dan variabel antara adalah DHT, TLR-2, dan IL-8. Hasil : Lesi AV pada awal penelitian pendahuluan 100,178,92 dan akhir penelitian 78,945,85 terdapat penurunan bermakna (t:2,525)(p:0,019). Delta kelompok isoflavon 160 mg lebih besar dari plasebo, isoflavon 40 mg, 80 mg, dan 120 mg (Z:-2,611)(p: 0,009). Lesi AV pada awal penelitian lanjutan 97,8547,614 dan akhir penelitian 59,3845,549, terdapat penurunan yang bermakna (Z:-4,300)(p:0,000). DHT awal penelitian 307,6150,38 pg/ml dan akhir penelitian 283,5253,13 pg/ml, terdapat penurunan yang bermakna (Z:-2,204)(p:0,027). TLR-2 awal penelitian 4539,82862 pg/ml dan akhir penelitian 3944,63592,42 pg/ml terdapat penurunan bermakna (Z:-3,624)(p:0,000). IL-8 awal penelitian 411,3167,137 pg/ml dan akhir penelitian 311,09103,917 pg/ml, terdapat penurunan bermakna (Z:-4,557) (p:0,000). Simpulan : Pemberian suplementasi isoflavon kedelai dengan variasi dosis selama 4 minggu, dapat menurunkan lesi AV dan diperoleh dosis yang paling baik yaitu dosis 160 mg serta pemberian selama 12 minggu menyebabkan penurunan bermakna terhadap lesi AV, DHT, TLR-2, dan IL-8 dibandingkan terapi standar. Kata kunci : Akne vulgaris, isoflavon kedelai, lesi AV, DHT, TLR-2, IL-8. Background. Acne vulgaris (AV) is the most common feature of skin inflammatory diseases. Soy isoflavone has proven as an anti-androgen and an anti-inflammation. The purpose of this research was to prove the effect of soy isoflavone in AV lesion that caused by the decreased level of dihydrotestoreone (DHT), Toll-like receptor-2 (TLR-2), Interleukin-8 (IL-8) in women with AV. Methods. This research used randomized pre and post test control design. The first research had 25 samples, randomized in 5 groups: placebo, isoflavone 40 mg, 80 mg, 120 mg, 160 mg group in 4 weeks length of research. The last research had 40 samples, randomized in controled and threated group in 12 weeks length of research. The independent variables was soy isoflavone, the dependent variables was AV, the confounding variables were DHT, TLR-2, and IL-8. Results. There was a significant degradation (t=2.525; p=0.019) of AV lesion in the early (100.1+78.92) and in the end of the first research (78.9+45.85). Delta of isoflavone 160 mg group more than other groups (Z=-2.61; p=0.009). There was a significant degradation (Z=-4.300; p=0.000) of AV lesion in the early (97.85+47.614) and in the end of the last research (59.38+45.5). DHT level had a signficant decrease (Z=-2.204; p=0.027) from the first research (307.6+150.38 pg/ml) to the last research (283.5+253.13 pg/ml). TLR-2 level had a signficant decrease (Z=-3.624; p=0.000) from the first research (4539.8+2862 pg/ml) to the last research (3944.6+3592.42 pg/ml). IL-8 level had a signficant decrease (Z=-4.557; p=0.000) from the first research (411.31+67.137 pg/ml) to the last research (311.09+103.917 pg/ml). Conclusion. The administration of soy isoflavone with variances of doses in 4 weeks can decrease AV lesion and the best dose was 160 mg, and the administration in 12 weeks can make significant degradation of AV lesion and significant decreased level of DHT, TLR-2, and IL-8 compared by standard therapy. Keywords: Acne vulgaris, soy isoflavone, AV lesion, DHT, TLR-2, IL-8