PENGARUH PEMBERIAN TERAPI MINYAK Nigella sativa DAN KOMBINASINYA DENGAN SEFTRIAKSON TERHADAP JUMLAH KUMAN Methicillin Resistant Staphylococcus aureus (MRSA) PADA KULTUR LIMPA MENCIT BALB/c

Abstract

Background Methicillin Resistant Staphylococcus aureus (MRSA) is a strain of Staphylococcus aureus which is resistant to metichilin and another antibiotics. Researchs are being developed to find new therapies for MRSA infection. Nigella sativa has an antibacterial effect. Effect of Nigella sativa on the number of MRSA bacteria in vivo have not been investigated. Aims To prove the effect of Nigella sativa oil and its combination with ceftriaxone toward MRSA count in BALB/c mice’s spleen culture. Methods This was true laboratory experimental study with post test only control group design. The samples were 20 males BALB/c mice, randomized into 4 groups. Control was given 0,03 ml aquabidest injected intraperitoneally, P1 was given 0,03 ml ceftriaxone injected intraperitoneally, P2 was given 0,3 ml Nigella sativa oil orally and P3 was given the combination of 0,03 ml ceftriaxone and 0,3 ml Nigella sativa oil. 0,2 ml (107 cfu/ml) MRSA was injected intraperitoneally at the hour 0. The treatment was given at the hour 16 and mice were terminated at the hour 24. Kruskall-Wallis Test and Mann-Whitney Test is used to analyze the data. Results Results of the mean number of MRSA bacteria in spleen culture were K=124x103  160,70x103; P1=24,08x103  26,53x103; P2=0,78x103 1,52x103; P3=6,05x103  13,38x103. The number of MRSA bacteria decreased significantly between control group compared to P2 (p=0,016) and P3 (p=0,046) and P1 compared to P2 (p=0,016) and P3 (p=0,047). There were no significance between control compared to P1 (p=0,674) and P2 toward P3 (p=0,596). Conclusion Administration of Nigella sativa oil and its combination with ceftriaxone can decrease the number of MRSA bacteria in BALB/c mice’s spleen cultures. Key words : MRSA, Nigella sativa, ceftriaxone, in vivo