PENGARUH PEMBERIAN EKSTRAK BUAH KERSEN (MUNTINGIA CALABURA L.) DOSIS BERTINGKAT TERHADAP GAMBARAN HISTOPATOLOGI HEPAR MENCIT BALB/C YANG HIPERURISEMIA

Abstract

Background: Cherry has often been used by people as a traditional medicine. A benefit of cherry is to reduce uric acid level. Liver is a central organ that acts to metabolize foreign substances which enter the body, so the toxic effects of medication usually show in the liver, especially the changes of histopathological images of liver. Aim: To prove the differences of histopathologic images of hyperurisemic Balb/c mice’s liver between the groups of mice given cherry extract with graded dosages and the control groups of mice and the differences between pre test uric acid level and post test uric acid level. Methods: This was a true-experimental research study using the pre test and post test control group design. 30 Balb/c mice were divided into 6 groups randomly. The control 1 which was given only standard food, control 2 was given high purine diet, control 3 was given cherry extract, and treated groups (P1, P2, P3) which were given cherry extract in graded dosage: 14 mg/20grBW, 28 mg/20grBW, and 56mg/20 grBW for 30 days. On day 31, the uric acid level were measured , then the mice were terminated. The liver were sliced and stained using HE stain. The liver of Balb/c mice were examined under microscope in five viewing fields the were compared between the control groups and treatment groups. Results: One Way ANOVA test for histopathological images of degeneration liver showed insignificant differences (p>0,05) for every group. Wilcoxon test for uric acid level showed significant differences (p<0,05) for every group. Conclusion: There were not significant difference between cherry extract with graded dosages to histopathological images of hyperuricemia Balb/c mice’s liver. But there were significant differences for reduction of mices’ uric acid level. Keywords: cherry, muntingia, liver, uric acid.