PENGARUH PEMBERIAN SUSU KAMBING TERHADAP GAMBARAN MIKROSKOPIS TESTIS DAN KADAR TIMBAL (Pb) DALAM DARAH TIKUS WISTAR YANG TERPAPAR ASAP KENDARAAN BERMOTOR

Abstract

Background: Testis is the most important male reproductive organ. Lead (Pb) which is produced by incomplete fuel combustion on motorized vehicle can inhibit spermatogenesis process and increase infertility risk factor. Goat milk is a natural substance which contains of flavonoid, vitamin C, E, B1, B6, B9, Ca, Zn, Mg, Fe and useful as an antioxidant, Pb chelating agent and Pb absorption inhibitor into bloodstream. A decrease of Pb blood level influence microscopic appearance of testicle seminiferous tubules which related with spermatogenesis process. Aim: To verify the effect of goat milk on microscopic testes appearance and Pb blood level of wistar rats with motorized vehicle emission exposure. Method: This was a true experimental research study with post-test only controlled group design. The sample were 15 wistar rats, 2-3 month old, 100 –200 gram. The sample were divided into 3 groups randomly, namely control group (K) which was given standard food and beverage only, treated group (P1,P2) which was given motorized vehicle emission exposure 8 hour/day for 30 days and 473.2mg/kgBW goat milk for P2.On day 31, blood and testes were taken and the rats were terminated, then examined Pb blood level and microscopic seminiferous tubule testes appearance of wistar rats each groups. Result: The administration of goat milk before motorized vehicle emission exposure on P2 group had less the mean of Pb blood level P2(7,80μg/dl± 4,18265)<P1 (11,24μg/dl± 6,27229)(p>0.05) while the seminiferous tubule microscopic damage P2<P1(p>0.05). Conclusion: Goat milk 473,2mg/kgBB does not effect on Pb blood level and seminiferous tubules testis damage prevention caused by motorized vehicle emission exposure significantly Keyword : goat milk, lead, Pb, microscopic, testis, seminiferous tubules, vehicle emission exposure