PENGARUH PEMBERIAN SUSU KAMBING TERHADAP GAMBARAN MIKROSKOPIS PARU DAN KADAR HEMOGLOBIN (Hb) TIKUS WISTAR YANG TERPAPAR ASAP KENDARAAN BERMOTOR

Abstract

Background Lung is an organ respiratory and may occur abnormalities associated with inflammatory caused by an exposure to smoke from motor vehicles. Smoke from motor vehicles can also cause anemia because it increases rate of hemolysis and inhibits synthesis of hemoglobin. Goat's milk is rich of functional components with antioxidant properties that can prevent lung inflammation and anemia because of smoke from motor vehicles. Aim To prove the effect of goat’s milk on the difference of microscopic appearance of lung and hemoglobin level of Wistar rats exposed to smoke from motor vehicles. Methods This study was a true experimental study with post-test only controlled group design. Samples were 15 Wistar rats, aged 2-3 months, divided into 3 groups: control group (K), was only given standard food and drink; treatment group 1 (P1), was exposed to smoke from motor vehicles for 8 hours per day for 30 days long; and treatment group 2 (P2), was given goat’s milk with 473.2 mg / KgBW dose and exposed to smoke from motor vehicles for 8 hours per day for 30 days afterwards. On day 31st, the mice blood drawn through retroorbita then terminated for change in microscopic appearance of lung observed. Results There was significant difference of microscopic appearance of lung from Mann Whitney test (p <0.05), groups of K-P1, P1-P2 (p = 0.004, p = 0.004) whereas K-P2 was found insignificant (p = 0.072). Besides, the mean hemoglobin level P1(13,1±0,233) was higher than K (12,9±0,949) with (p>0.05) and P2 (14,6±0,464) was higher than P1 and K (p <0.05). Conclusion Goat's milk inhibits lung microscopic damage include alveolar edema, destruction interalveolaris septum, and infiltration of inflammatory cell Wistar rats exposed to smoke from motor vehicles. Hemoglobin levels were higher in group Wistar rats were fed goat’s milk. Key words: Goat’s milk, microscopic, lung, smoke from motor vehicles 1) Student of Faculty of medicine Diponegoro University, Semarang 2) Staff on Histology departement faculty of medicine Diponegoro University, Semarang