UJI BEDA SENSITIVITAS KANAMISIN DENGAN SEFTRIAKSON PADA KUMAN NEISSERIA GONORRHOEAE SECARA IN VITRO

Abstract

Background: Incidence of gonorrhe tends to increase across the world. The sensitivity of Ceftriaxone as first line treatment agent for patients with gonorrhe has been declining, hence alternative treatment is needed. Kanamycin is one of the proposed second line treatment agent for gonorrhe. Purpose: To assess the difference in sensitivity of Neisseria gonorrhoeae towards Kanamycin and Ceftriaxone in vitro Materials & Methods: A cross sectional study which examined 13 subjects with positive purulent discharge, gram staining found negative gram diplococcic bacteria, followed by a Thayer-Martin culture and a 24-hours incubation on 370C temperature. The colony samples were then moved to a Mueller Hinton media for Kanamycin and Ceftriaxone sensitivity testing, the inhibition zone formed on the colony samples was measured. Results: Culture samples of Neisseria gonorrhoeae which declared sensitive towards Kanamycin were 10 (76.9%), and those which declared sensitive towards Ceftriaxone was only 1 (7.7%), with p < 0.05 Conclusion: This study shown a significant difference between sensitivity of Neisseria gonorrheae towards Kanamycin and Ceftriaxone, with greater sensitivity towards Kanamycin.. Keywords: Neisseria gonorrhoeae, kanamycin, ceftriaxone, minimum inhibitory zone, sensitivity