DEPOLIMERISASI KITOSAN DENGAN HIDROLISA ENZIMATIK MENGGUNAKAN ENZIM α-AMILASE

Hanik, Handayani P. L. and Paramarta, Siwi R and Nur, Rokhati (2013) DEPOLIMERISASI KITOSAN DENGAN HIDROLISA ENZIMATIK MENGGUNAKAN ENZIM α-AMILASE. Jurnal Teknologi Kimia dan Industri, 2 (4). pp. 55-64.

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Abstract

Chitosan is a polymer derivative of chitin that has many benefits in food and medical. Since chitosan has a large molecular weight, it has constraints to its application. To overcome the disadvantage, it needs hydrolysis of chitosan in order to obtained a lower molecular weight. In this research the hydrolysis of chitosan will be operated by enzymatic process uses thermophilic enzyme α-amylase. The experiment begins by making a chitosan solution 1% w/v in an acetic acid solution 1% v/v. The chitosan solution is hydrolyzed using the enzyme α-amylase with varied pH, operating temperature, time, and ratio of the enzyme:substrate to obtain the optimum. The response comprehends Dextrose Equivalent (DE), viscosity, and the average of molecular weight. The results showed that the optimum operating conditions for chitosan hydrolysis using α-amylase enzyme is at pH 5, temperature 90 °C, 2 hours operating time, and ratio of enzyme:substrate 1:2500 (0,1 ml). For variable pH, temperature, time, and enzyme addition, DE rises meanwhile variables are increased, then it dropped significantly after the optimum level. The hydrolysis can reduce molecular weight of chitosan from 1680-1750 kDa to 144,18 kDa thus it is included to MMWCs.

Item Type:Article
Uncontrolled Keywords:Chitosan; Hydrolysis; Enzyme α-amylase; Dextrose Equivalent; Molecular weight
Subjects:T Technology > TP Chemical technology
Divisions:Faculty of Engineering > Department of Chemical Engineering
Faculty of Engineering > Department of Chemical Engineering
ID Code:41485
Deposited By:teknik kimia
Deposited On:22 Jan 2014 01:05
Last Modified:22 Jan 2014 01:05

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