PENGARUH FORMALIN PERORAL DOSIS BERTINGKAT SELAMA 12 MINGGU TERHADAP GAMBARAN HISTOPATOLOGIS ESOFAGUS TIKUS WISTAR

Abstract

Background: Recently there are so many foods which are not healthy for our body. These are proven by founding an experimental declaring that formalin was found in a sample food. Formalin is an addition substance which is forbidden to be used in a food composition because it gives carcinogenic effect and brings harm effect to our health. Formalin which is ingested can give some effects to all body system included gastrointestinal tract. We will discuss about esophagus in our laboratory experimental. Aim: To prove the effect of gradually per oral dosage of formalin to the change of histopathology image of wistar rat esophagus. Method: This research was conducted using laboratory experiment with Post test only control group design. The samples were 20 wistar rats which had fulfilled the inclusion and exclusion criteria and then adapted for seven days. After completed the adaptation period,wistar rats were divided using simple random sampling into 4 groups. K is control group without per oral formalin dosage. P1 was given formalin 50mg/kgW/day, P2 was given formalin 100mg/kgW/day, and P3 was given formalin 200mg/kgW/day. After 12 weeks, all esophagus sample were taken out to identify histopathology changes. Data was described in table and images, statistics analysis were done using computer program. Result: The highest rate of esophagus mucose damage is on group P3. By non parametric test Kruskal-Wallis it was got a significant difference (p=0,000). Then after it was analyzed using Post Hoc (Mann-Whitney), found that there was significant differences in K-P1 (p=0,008), K-P2 (p=0,006), K-P3 (p=0,007), P1-P2 (p=0,009), P1-P3 (p=0,008), P2-P3 (p=0,006). Conclusion: Gradual per oral formalin dose for 12 weeks caused histopathological changes on wistar rat’s esophagus. The changes showed including epithelial desquamation, epithelial erosion and epithelial ulceration. Keywords: Gradual dose of formalin, histopathological image of esophagus