DARAH MANUSIA YANG DICUCI SEBAGAI ALTERNATIF MENINGKATKAN KEMAMPUAN MENUMBUHKAN : Streptococcus pneumoniae PADA MEDIA AGAR DARAH

Abstract

Background Sheep Blood Agar (SBA) is a standard media for microbiology examination. The difficulty to get sheep blood in tropical and developing countries causes an obstacle in culturing S. pneumoniae. In other side, the usage of Human Blood Agar (HBA) were not recommended for culturing S. pneumoniae. Aim To modify HBA by washing human erythrocytes three times to improve the growth of S. pneumoniae. Methods Experimental study with True experimental post test only design. Nine strains of S. pneumoniae were suspensed into sputum and then were planted on three media HBA, SBA, and washed human blood agar (WHBA). Observation was done in 24 hours and 48 hours, comprising of colony diameters, hemolysis diameters, and the characteristics colony. Results After 24 hours, the mean of S. pneumoniae colony diameters on WHBA(0,9±0,26 mm) and SBA were not significantly different (0,8±0,09 mm; p=0,173) and those on HBA (0,6±0,10 mm) were narrower than those on SBA and WHBA (p=0,000). On the 48 hours observation, the mean of colony diameters on WHBA (1,4±0,23 mm) and HBA (1,5±0,13 mm) were narrower than those on SBA (1,8±0,10 mm; p=0,000). After 24 hours, the mean of S. pneumoniae hemolysis diameters, WHBA (1,2±0,34 mm) and SBA (1,2±0,14 mm) were not significantly different (p=0,738) and those on HBA (0,6±0,15 mm) were narrower than those on SBA and WHBA (p=0,000). After the 48 hours observation, hemolysis diameters were widest in WHBA (1,8±0,34 mm) followed by SBA (1,6±0,07 mm p=0,008) and in HBA (0,8±0,15 mm; p=0,000). The characteristics of colony in 24 and 48 hours observation were not significantly different in all media (p=0,083). Conclusion WHBA is an acceptable alternative media for culturing S. pneumoniae in poorly resourced laboratories. Key Words: S. pneumoniae, WHBA, SBA, HBA