THE EFFECT OF TRANS FATTY ACID DIET ON BLOOD GLUCOSE LEVEL OF WISTAR RATS (A PRELIMINARY STUDY)

Abstract

Background: Trans fatty acids are unsaturated fatty acids that contain at least one double bond in the trans configuration and formed through the process of partial hydrogenation. It is suggested that trans fatty acid may increase the risk of the development of type 2 diabetes mellitus by worsening insulin resistance, thus increasing the blood glucose level. This study is aimed to identify and analyze the effect of trans fatty acid diet on blood glucose level of wistar rats. Method: A Series of experiment using Pre and Post Test Randomized Controlled Group Design was carried out in six wistar rats which were divided into two groups, one group was control group, received standard diet and the other was intervention group, received additional orally 10 gr/day margarine containing 0.8% (of total energy) trans fatty acids per gram margarine. The blood sampling was obtained from the retro-orbital plexus before and after intervention to determine the blood glucose level of each wistar rats. Blood glucose level was measured with “GOD-PAP” method. The experiment was given for two weeks. Result: Paired sample T-Test with p=0.056, showed there was an insignificant increment of blood glucose level in rats which have been given TFA diet for two weeks. The result of independent sample T-Test for control group and intervention group, p=0.658 has shown that the increment of blood glucose level between control group and intervention group was insignificant. Conclusion: Intake of 10 gr/day margarine containing 0.8% (of total energy) trans fatty acids per gram margarine for two weeks did not significantly induce the increment of blood glucose level of wistar rats. Key Words: Trans fatty acid, blood glucose level