OPTIMALISASI AGAR COKLAT DARAH MANUSIA SEBAGAI MEDIA UJI SENSITIVITAS ANTIBIOTIK TERHADAP Haemophilus influenzae: PERAN PACKED RED CELL

Abstract

Background: The standard medium for antimicrobial susceptibility testing of Haemophilus influenzae is Haemophilus Test Medium (HTM). It is difficult to provide and expensive, causing standard routine test for Haemophilus influenzae is very rarely done. In Indonesia the use of human blood as a medium for antimicrobial susceptibility testing of Haemophilus influenzae has not been done. Aim: To compare the agreement of the results of antimicrobial susceptibility testing of H.influenzae in ACD, ACMSt, and ACMH, with HTM to find an alternative medium for antimicrobial susceptibility testing of H.influenzae as HTM Methods: The design study used a True experimental post-test only. Antimicrobial susceptibility test was carried on by disc diffusion method (CLSI 2011) on HTM, ACD, ACMSt, ACMH against 11 strains of H.influenzae. Data were analyzed by Kappa agreement test with the minimum agreement k> 0.80 (very good agreement). Results: For ACD, k> 0.80 were only achieved by 40% antibiotic tested (chloramphenicol and cotrimoxazole). For ACMSt, very good agreement was achieved only by 60% antibiotic tested (chloramphenicol, cotrimoxazole, and tetracycline). For ACMH, very good agreement was achieved by all(100%) of the antibiotics tested (chloramphenicol, co-trimoxazole, tetracycline, amoxicillin-clavulanic acid, and ceftriaxone). Conclusion: ACMH could be used as an alternative medium for antimicrobial susceptibility testing of H.influenzae. Keywords: Haemophilus influenzae, hemoglobin, chocolate agar, human blood, antimicrobial susceptibility testing