PENGARUH SUPLEMENTASI EKSTRAK DAUN ANNONA MURICATA TERHADAP KEJADIAN DISPLASIA EPITEL KELENJAR PAYUDARA TIKUS SPRAGUE DAWLEY YANG DIINDUKSI 7, 12 DIMETHYLBENZ[α]ANTHRACENE

Abstract

Background: Breast cancer is the most common cancer in women worldwide. Annona muricata has long history as an alternative therapy for cancers in society. There has no been scientific literature about the effect of Annona muricata in carcinogenesis induced by 7,12 Dimethylbenz[a]nthracene (DMBA). This study is aimed to investigate the effect of Annona muricata leaves extract suplementation on the dysplasia incidence of mammary glands epithel of Sprague Dawley rats. Methods: This study utilized randomized post test only control group design. Fifteen rats were divided into 3 groups (K, P I, P II). All groups were induced by DMBA intragastric twice a week for 5 weeks. After having a rest for 3 days post DMBA induction, P I group received Tamoxifen 0.18 mg and P II received Annona muricata leaves extract 200 mg/kg body weight for 8 weeks. The dysplasia incidence was evaluated based on the percentage of ductal and lobular dysplasia. Result: The lowest percentage of both dysplasias was on group P II. One way ANNOVA test presented significant differences (p<0.05) with p=0.021. According to Bonferroni post hoc test, there were significant differences between groups K and P II (p=0.027) for lobular dysplasia and between groups P I and P II (p=0.044) for ductal dysplasia. Conclusion: The present study demonstrates that the Annona muricata leaves extract suplementation can reduce the dysplasia incidence of mammary glands epithel of Sprague Dawley rats induced by DMBA. Keywords: Dysplasia, Annona muricata, DMBA, Breast cancer