PENGARUH PENINGKATAN KADAR HEMOGLOBIN TERHADAP PERTUMBUHAN Haemophilus influenzae PADA MEDIA AGAR COKLAT DARI DARAH MANUSIA

Abstract

Background: Haemophilus influenzae is a fastidious bacteria that causes meningitis and pneumonia. Chocolate agar made from sheep blood is the gold standard for cultivating media, but there is limitation in sheep blood availability in many countries. Human blood is cheaper and easy to provide, but not recommended for cultivating this bacteria. This research aimed to find the alternative media made from human blood by modification in enhanced hemoglobin level. Methods: This research was true experimental post test only study. Eleven strains were cultivated on sheep blood derived chocolate agar (SCA), human blood derived chocolate agar (HCA), and Hb modified human blood derived chocolate agar (HCA-Hb). Growth was evaluated on 24 and 48 hour based on diameter of the colonies, colony count, and characteristic of the colonies. In addition the strains were spiked into sputum and cultured on the same types of media. Growth was evaluated based on diameter and characteristic of colonies. Result: After 24 and 48 hours incubation, diameter of colony from pure suspension of H. influenzae on HCA-Hb was bigger that that on HCA (p>0,05), but smaller than that on SCA (p<0,05). However, diameter colony of H. influenza which was spiked into sputum and cultured on HCA-Hb was similar to that on SCA (p=0,064) and bigger than that on HCA (p=0,002). There was no differences of colony and characteristics on all media type (p>0,05). Conclusion: Chocolate agar made from human blood and modified by enhanced hemoglobin level can be used as an alternative media to cultivate H. influenzae. Keywords: Haemophilus influenzae, hemoglobin, chocolate agar, human blood