PENGARUH PEMBERIAN METANOL DAN ETANOLTERHADAP TINGKAT KERUSAKAN SEL HEPAR TIKUS WISTAR

Abstract

Background : Methanol usually used for organic solvent is a kind of alcohol that has the most simple structure but very toxic to human. Methanol intoxication can lead to acidosis metabolic, neurological complication, even death. Methanol and ethanol is metabolised mostly in liver and have potential to damage liver cell. There are lot of study about the effect of methanol and ethanol intoxication on liver cell but the study about the damage with microscopic analysis has not yet been found . This study aims to determine the histopathological effect of methanol and ethanol administration to liver cell damage in Wistar rat. Method : Experimental research with post test only controlled group design. Our sampel is 20 male Wistar rats that devided by one control and three treatment group. Treatment was gived through sonde with 6 ml/kg body weight per oral dose of methanol, 8gr/kg body weight dose of taurine. K group not recieved any treatment. P1 group recieved ethanol treatment. P2 recieved methanol treatment. P3 group recieved methanol and ethanol treatment. All the treatment is given for 14 days. After the treatment the rats was decapitated, autopsied the brain organ for preparation to make histopathology sampel. Result : Data result is analysed by One-way Annova test showed p=0,000. Continued by Post Hoc test showed that there is a significant differences between K-P1(p=0,002), K-P2(p=0,004), K-P3(p=0,000), P1-P3(p=0,029), P2-P3 (p=0,021). Conslusion : Methanol and ethanol have almost same destruction effect in the liver cell but not significant. However if they gived simultaneously methanol and ethanol have synergistic effect that both compound increased more damage in liver cell significantly compare to other treatment group Keywords : methanol, ethanol, wistar rat