A Simple Method for Efficient Extraction and Separation of C-phycocyanin from Spirulina platensis

Noer , Abyor Handayani and H, Hadiyanto and Melinda , Deviana and Inggar , Dianratri and Amin , Nugroho (2012) A Simple Method for Efficient Extraction and Separation of C-phycocyanin from Spirulina platensis. In: International Conference on Chemical and Material Engineering 2012. ISBN : 978-602-097-281-7, September 12 – 13, 2012, , Grand Candi Hotel, Semarang Indonesia.

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Abstract

Stroke is the third leading cause of death after cardiovascular disease and cancer. At present, there is no registered neuroprotective drug used in clinical practice that may prevent or limit cerebral tissue lesions occurring in the areas with decreased blood flow. C-Phycocyanin (C-PC) is a biliprotein pigment that plays an essential light-harvesting role in cyanobacteria with multiple applications in industry. Furthermore, phycocyanin has been proven to have therapeutic properties including antioxidant, neuroprotective agent, anti-inflammatory and anti-cancer activities. In this study, the C-PC was extracted by using sodium dihidrogen phophat (phosphate buffer). In this study, the effect of temperature and ratio of biomass and solven were also investigated. Extracted C-PC showed absorbance maximum at 30°C, however extracted C-PC showed absorbance minimum at 70°C. The use of biomass and solvent ratio, 1:50, could extract the C-PC as well as the use of ratio 1:75, 1:100. Meanwhile, extraction process by using ratio of biomass : solven, 1:125, gave the lowest absorbance value of C-PC. Pure C-PC was finally obtained from Spirullina with purity ratio 1,19 – 1,42, and is potential to be applicated in cosmetic industries.

Item Type:Conference or Workshop Item (Paper)
Uncontrolled Keywords:C-phycocyanin, extraction, phosphate buffer, spirulina platensis
Subjects:T Technology > TP Chemical technology
Divisions:UNDIP Conference/Seminar > International Conference on Chemical and Material Engineering 2012
Faculty of Engineering > Department of Chemical Engineering
Faculty of Engineering > Department of Chemical Engineering
ID Code:36938
Deposited By:teknik kimia
Deposited On:15 Nov 2012 01:43
Last Modified:15 Nov 2012 01:43

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