A Simple Method for Efficient Extraction and Separation of C-phycocyanin from Spirulina platensis

Abstract

Stroke is the third leading cause of death after cardiovascular disease and cancer. At present, there is no registered neuroprotective drug used in clinical practice that may prevent or limit cerebral tissue lesions occurring in the areas with decreased blood flow. C-Phycocyanin (C-PC) is a biliprotein pigment that plays an essential light-harvesting role in cyanobacteria with multiple applications in industry. Furthermore, phycocyanin has been proven to have therapeutic properties including antioxidant, neuroprotective agent, anti-inflammatory and anti-cancer activities. In this study, the C-PC was extracted by using sodium dihidrogen phophat (phosphate buffer). In this study, the effect of temperature and ratio of biomass and solven were also investigated. Extracted C-PC showed absorbance maximum at 30°C, however extracted C-PC showed absorbance minimum at 70°C. The use of biomass and solvent ratio, 1:50, could extract the C-PC as well as the use of ratio 1:75, 1:100. Meanwhile, extraction process by using ratio of biomass : solven, 1:125, gave the lowest absorbance value of C-PC. Pure C-PC was finally obtained from Spirullina with purity ratio 1,19 – 1,42, and is potential to be applicated in cosmetic industries.