The Determination Of Salinity Profile And Nutrition (Nah2po4) Profile In Utilizing Nannochloropsis oculata To Gain Maximum Lipid

Abstract

The determination of salinity profile and nutrition (NaH2PO4) profile in utilizing Nannochloropsis oculata to gain maximum lipid was investigated. The purpose of carrying out this research is to determine the optimum salinity and the optimum nutrition (NaH2PO4) to gain maximum lipid contained in Nannochloropsis oculata. Phosphor as a nutrition can be obtained by adding NaH2PO4 and a salinity enhancement can be applied by adding NaCl to the culture.This research was run by using variable of salinity (33, 34, 35, and 36 ppt) and variable of NaH2PO4 (5, 10 and 15 ppm). The results show that the percentage of maximum lipid in Nannochloropsis oculata was obtained when the salinity of 35 ppt and 5 ppm NaH2PO4 is 37,68%. However, this maximum lipid percentage is low and it can be caused by many factors, such as the drying temperature for preparing the dry microalgae to extract, the cell disruption method and the extraction solvent used in the research. By seeing the result, salinity can affect the total lipid contained in Nannochloropsis oculata. The higher the salinity contained in culture the higher the lipid contained in Nannochloropsis oculata. Otherwise, a different respond was obtained by adding NaH2PO4. If NaH2PO4 concentration is higher, the lipid contained Nannochloropsis oculata will be lower.