Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir

Akbarningrum , Fatmawati and Rudy , Agustriyanto and Rudy , Agustriyanto and Carolina , Adhelia and Yusnita , Liasari (2012) Enzymatic Hydrolysis of Alkaline Pretreated Coconut Coir. In: International Conference on Chemical and Material Engineering 2012. ISBN : 978-602-097-281-7, September 12 – 13, 2012, , Grand Candi Hotel, Semarang Indonesia.

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Abstract

The purpose of this research is to study the effect of concentration and temperature on the cellulose and lignin content, and the reducing sugars produced in the enzymatic hydrolysis of coconut coir. In this research, the coconut coir is pretreated using 3%, 7%, and 11% NaOH solution at 60oC, 80oC, and 100oC. The pretreated coir were assayed by measuring the amount of cellulose and lignin and then hydrolysed using Celluclast and Novozyme 188 under various temperature (30oC, 40oC, 50oC) and pH (3, 4, 5). The hydrolysis results were assayed for the reducing sugar content. The results showed that the alkaline delignification was effective to reduce lignin and to increase the cellulose content of the coir. The best delignification condition was observed at 11% NaOH solution and 100oC which removed 14,53% of lignin and increased the cellulose content up to 50,23%. The best condition of the enzymatic hydrolysis was obtained at 50oC and pH 4 which produced 7,57 gr/L reducing sugar.

Item Type:Conference or Workshop Item (Paper)
Additional Information:ISBN : 978-602-097-281-7
Uncontrolled Keywords:coconut, enzyme, hydrolysis, lignocellulose
Subjects:T Technology > TP Chemical technology
Divisions:UNDIP Conference/Seminar > International Conference on Chemical and Material Engineering 2012
Faculty of Engineering > Department of Chemical Engineering
Faculty of Engineering > Department of Chemical Engineering
ID Code:36872
Deposited By:teknik kimia
Deposited On:13 Nov 2012 23:14
Last Modified:15 Nov 2012 23:12

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