SALTING OUT MANNAN LIDAH BUAYA (ALOE VERA) MENGGUNAKAN CO-SOLVENT METANOL, ETANOL, DAN ISOPROPANOL

Abstract

Aloe vera has a good antimicrobial ability, it can even inhibit the growth of pathogenic microbes such as Escherichia coli, Micrococus luteus, Staphylococcus aureus and several other microbial pathogens. Mannan compounds are found in aloe vera, particularly acetylated mannan or acemannan Mannan able to boost immunity and effective against the virus causes flu and measles. Extraction - precipitation process (salting out) is a way to take an active substance (acemannan) contained in aloe vera. Fixed variables use In this process are 400 grams of Aloe vera, extraction temperature at 300C, and the weight ratio (gr) between Aloe vera and water as solvent is 1:4. While variables used in this research are type of co-solvent alcohol (methanol, ethanol and isopropanol), and volume ratio between co-solvent and filrat from the juicer and extraction (2.5, 3, 3.5, and 4). Based on research results obtained that, isopropanol is best co-solvent used in mannan precipitation, and the greater volume ratio of co-solvent then the greater yielded Mannan.