Hepatoprotective effects of Nigella sativa seeds extract against ethanol induced hepatic tissue changes in Wistar rats

Mohamed Jaber, Zaid (2012) Hepatoprotective effects of Nigella sativa seeds extract against ethanol induced hepatic tissue changes in Wistar rats. Masters thesis, Diponegoro University.

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Official URL: http://mbiomedik.undip.ac.id/

Abstract

Background: It has been reported that Nigella sativa seed extract posses a hepatoprotective effect in liver toxicity models, additionally has been used in the treatment of liver disease in traditional medicine. Goal: The aim of the study was to investigate the hepatoprotective effects of Nigella sativa seed extract towards ethanol-induced hepatic tissue changes. Method: The design of the study was a post test control group design. Twenty four Wistar rats were randomly allocated into four groups; the first group treated with ethanol only, group 2, 3, and 4 were given extracts of Nigella sativa seed intragastrically with the dose of 0.5, 1 and 1.5 g/kg b.w/day respectively followed by ethanol 8g/kg b.w/day for 8 weeks. Hepatic tissue changes were stained by HE and analyzed by Kruskal-Wallis and Mann-Whitney tests. Meanwhile, Mallory bodies were analyzed by Kendall's tau-b test. Result: The liver tissue in the control group showed a remarkable hepatic steatosis, hepatic inflammation and the present of Mallory bodies. There were significantly less difference between all groups, with p=0. 000, for both of hepatic steatosis and hepatic inflammation respectively. However there was no significant difference of hepatic steatosis between group 1 and control group (p=0.241). There was also a significant association (p=0. 001) between Nigella sativa seed extract and none presence Mallory bodies in treatment groups. Conclusion: Nigella sativa seed extract has a hepatoprotective effect towards ethanol induced hepatic tissue changes. The optimal dose of extract was 1.5g/kg B.w. Keywords: Nigella sativa seed extract; hepatic steatosis; hepatic inflammation; Mallory bodies

Item Type:Thesis (Masters)
Subjects:R Medicine > R Medicine (General)
Divisions:Postgraduate Program > Master Program in Biomedical Science
ID Code:35609
Deposited By:Mr. Magister Biomedik Admin
Deposited On:22 Jun 2012 09:06
Last Modified:22 Jun 2012 09:06

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