STUDY EFFECT OF CURCUMA LONGA RHIZOME EXTRACT ON LIVER CELL AFFECTED BY PASSIVE SMOKING Expiremental study in Sprague Dawley Rats

Abstract

Background: damage effect of the passive smoking in liver tissue range from liver cell changes to liver tissue inflammation,Curcuma longa rhizoma shows anti-inflammatory effect and antioxidant,however there is no literature about its regenerative effect . Objective: to identify and analyze the regenerative effect of Curcuma longa rhizome extract on passive smoking induced liver damage in SD Rats. Methods: This experimental study used randomized post-test only control group design in 24 SD Rats given 5 cigar as passive cigarette smoking with 50 minute as interval time between each cigaratte daily for 10 weeks (groupA), 13 weeks (group B), 10 weeks (group C) + oral curcuma longa rhizoma exrtact with dose 80mg/.kg,B.W,after got passive smoke daily , 13 weeks (group D) + oral Curcuma longa rhizoma exrtact with dose 80mg/kg,B.W, after got passive smoke daily ,The liver tissues were HE stained and observed for liver cell change,and immunohistochemistry was undertaken to count the percentage of TNF-α stained according to Allred Score. Result: the difference among groups (A&C) were statistically significant (p=0.01). and between groups (B&D) statistically significant (p=0.003) regenirative effect for the liver cell changes ,the difference of TNF-α expression among groups (A&C)was non statistically significant (p=0.5), and between (B&D) groups were statistically significant (p=0.04). Conclusion: there was regenerative effect on liver cell changes in groups with Curcuma longa rhizoma extract and also there was regenerative effect on the TNF-α expression with Curcuma longa rhizome Key Words : Curcuma longa rhizoma extract, cigarette smoking, liver nucleaus nucleaus change, TNF-α expression