PROTECTIVE EFFECTS OF NIGELLA SATIVA EXTRACT ON ETHANOL INDUCED HEPATIC TISSUE DAMAGE AND TNFα EXPRESSION (Experimental Study In Wistar Rats)

M.S Nairat, Muath (2012) PROTECTIVE EFFECTS OF NIGELLA SATIVA EXTRACT ON ETHANOL INDUCED HEPATIC TISSUE DAMAGE AND TNFα EXPRESSION (Experimental Study In Wistar Rats). Masters thesis, Diponegoro University.

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Official URL: http://mbiomedik.undip.ac.id/

Abstract

Background: Alcohol intake causes fatty liver, liver tissue inflammation, and liver cirrhosis, Nigella sativa seeds had been reported with anti-inflammatory effect. Objective: To identify and analyze the protective effect of Nigella sativa extract on alcohol induced liver damage in wistar rats. Method: This experimental study used randomized post-test control group design in 24 male Wistar rats divided into four groups each group contain 6 rats, control group given ethanol 8mg/kgBW/day for 8 weeks, group1 ,group 2,group 3, given Nigella sativa extract plus ethanol after one hour for 8 weeks as 0.5 g/kgBW/day, 1 g/kgBW/day, 1.5 g/kgBW/day respectively, ethanol dose 8mg/kgBW/day, the liver tissue were H&E stained and observed for liver cell changes , Imunohistochemistry was done to count the percentage of TNFα in Cytoplasm stained brown color and the intensity of the staining then its classify according to Allred Score. Result: There was severe, moderate, mild and normal liver tissue for control, group1, group2,and group3 respectively the difference among groups and between each group were statistically significant (p=0.00), except between control group and group 1 (P=0.093) for the liver tissue damage, there was 3 Allred,2 Allred, 1 Allred and 0 Allred for control, group1, group2, group3 respectively the difference among groups and between all groups was statistically significant (p=0.00) for TNFα expression. Conclusion: Nigella sativa extract shows protective anti-inflammatory effect, as reducing the damage change and the TNFα expression in hepatocye that induced bu ethanol . Keywords: ethanol, liver tissue damage, TNFα

Item Type:Thesis (Masters)
Subjects:R Medicine > RB Pathology
Divisions:Postgraduate Program > Master Program in Biomedical Science
ID Code:35603
Deposited By:Mr. Magister Biomedik Admin
Deposited On:22 Jun 2012 08:31
Last Modified:22 Jun 2012 08:31

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