Characterization of Psychrotrophic Bacteria in the Surface and Deep-Sea Waters from the Northwestern Pacific Ocean Based on 16S Ribosomal DNA Analysis

Radjasa, Ocky Karna and Hidetoshi, Urakawa and Kumiko Kita, Tsukamoto and Kouichi, Ohwada (2001) Characterization of Psychrotrophic Bacteria in the Surface and Deep-Sea Waters from the Northwestern Pacific Ocean Based on 16S Ribosomal DNA Analysis. Marine Biotechnology, 3 . pp. 454-462.

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Official URL: http://dx.doi.org/10.1007/s10126-001-0050-1

Abstract

Seventy-eight 4°C-culturable bacteria were isolated using ZoBell 2216E medium from surface (0–200 m) and deep-sea (1000–9671 m) waters in the northwestern Pacific Ocean. Growth studies indicated that all 4°C-culturable bacteria were psychrotrophs. Six phylotypes were observed in the surface water samples and 8 phylotypes in the deep-sea waters. Phylogenetic characterization based on 16S ribosomal DNA sequence analysis of the representative phylotypes revealed that some bacterial genera, Pseudoalteromonas, Photobacterium, and Vibrio, were common to surface and deep-sea waters, and others, Pseudomonas and Halomonas, specifically occurred in surface water. Overall, the members of Vibrionaceae appear to be dominant in both habitats.

Item Type:Article
Uncontrolled Keywords:marine psychrotrophs, 16S rRNA, RFLP, Vibrionaceae, Vibrio, Photobacterium, Pseudoalteromonas.
Subjects:S Agriculture > SH Aquaculture. Fisheries. Angling
G Geography. Anthropology. Recreation > GC Oceanography
Divisions:Faculty of Fisheries and Marine Sciences > Department of Marine Science
ID Code:355
Deposited By:Mr. Sugeng Priyanto
Deposited On:23 Jul 2009 14:51
Last Modified:29 Jul 2009 15:02

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