SSCP Profiles of 165 rRNA Gene of Thermophilic Bacterial Communities Inhabiting the Local Hot Stream

Abstract

Identification of the bacterial communities from one hot stream at Gedongsongo (GS3), Ungaran, Central Java; was carried out using Single Strand Conformation Polymorphism (SSCP) method.Two minimal media, GYa and GTa were used to grow semi anaerobic microbial communities. Cultures media were combined by filtration through 0,2-µm-pore-size filter for total hot stream community. PCR amplifications of partial 16S rRNA gene sequences were performed with chromosomal DNA extracted from cells of filtration and cultures using primers targetting the V4-V5 region of 16S rRNA genes. These primers amplified about 400-bp section of the 16S rRNA genes. The SSCP profiles showed that there were five bands from filtration sample, two bands from GTa culture and three bands from GYa culture. All of bands represent different phylotypes.