Aminin, A.L.N. and Asyari, M. and Mulyani, N.S. and Madayanti, F. and Aditiawati, P. and Akhmaloka, Akhmaloka (2006) PHYLOGENETIC ANALYSIS OF BACTERIAL COMMUNITIES IN PANCURAN 7 BATURRADEN HOT SPRING. PROCEEDING ASEAN Biochemistry Seminar "Enzymes : Industrial and Medical Prospects" Surabaya, February 6-7, 2006 . pp. 33-43. ISSN ISBN 979-99675-1-1
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Abstract
The diversity of the bacterial communities supported by culturing and capturing through 0.2 m-pore-size filter was studied. The Pancuran 7 hot spring has temperature at around 52°C and pH 7. Community fingerprint analysis by denaturing gradient gel electrophoresis (DGGE) of the PCR-amplified highly variable V9 region of the 16S rRNA gene from the domain Bacteria was performed. Three distinct DGGE bands have been analyzed for phylogenetic relationship. The 16S rDNA sequence fragment analysis of these bands revealed a high relationship with Bacillus group, two of them have a high similarity with Anoxybacillus sp. and one of the single colony that grown at ½ LB medium closely related to Geobacillus lituanicus.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | phylogeny, DGGE, thermophilic |
| Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH301 Biology |
| Divisions: | Faculty of Science and Mathematics > Department of Chemistry |
| ID Code: | 35485 |
| Deposited By: | INVALID USER |
| Deposited On: | 05 Jun 2012 22:06 |
| Last Modified: | 05 Jun 2012 22:06 |
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