Efektivitas Konsentrasi Sorbitol dalam Medium Purifikasi dalam Menghasilkan Jumlah Sel Viabel pada Isolasi Sel Mesofil Daun Pegagan (Centella asiatica (L.) Urban).

Puspitasari, Ika and Hariyanti, Sri and Prihastanti, Erma (2006) Efektivitas Konsentrasi Sorbitol dalam Medium Purifikasi dalam Menghasilkan Jumlah Sel Viabel pada Isolasi Sel Mesofil Daun Pegagan (Centella asiatica (L.) Urban). Anatomi Fisiologi, XIV (2). pp. 30-38. ISSN 0854-5367

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Abstract

Research abaut used sorbitol on purification media for sum cell viable product and effective sorbitol consentration to highes sum viable cell isolation phase pegagan mesophyl cell. Randomized Complete Design with 6 treatmen and 4 block. Sorbitol consentration in purification media was treatmen 0 g/L, 25 g?L, 50 g/L, 75 g/L, 100 g/L and 125 g/L. Parameter was sum of total cell, sum of viabel cell and cell viability. The result of this experiment indicated that the sorbitol in purification media gave the significant effect on the sum cell viabel and cell viability. Effective sorbitol consentration was 25 g/L with obtain sum cell 13,19 x 107 cell/ml and cell viability 99,39%.

Item Type:Article
Uncontrolled Keywords:sorbitol, cell purification, cell viability, purifikasi sel, viabilitas sel
Subjects:Q Science > QP Physiology
Divisions:Faculty of Science and Mathematics > Department of Biology
ID Code:34515
Deposited By:Mr. Biologi Admin
Deposited On:07 Mar 2012 08:27
Last Modified:14 Mar 2012 15:56

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