PENGARUH PEMBERIAN EKSTRAK BATANG SARANG SEMUT (Myrmecodia Pendens Merr & Perry) TERHADAP EKSPRESI p21 DAN EKSPRESI ki67 PADA GALUR SEL KARSINOMA MAMMAE T47D

Abstract

Background : Empirical Approaches done a lot of Indonesian society as a component in the use of herbal anti-cancer, in the absence of the underlying research. Extract of batang sarang semut in recent years is widely used in the treatment of breast cancer without clinical and laboratoric supervision. Study in vitro on the anticancer potential of stem extracts of sarang semut conducted among others by looking at its effect on the expression of p21 and ki67 on T47D cell line. p21 is a tumor supressor protein that functions in the induction of cell cycle arrest and DNA repair. While ki67 is cell proliferation marker that is always present in every proliferation cell activity that is nutritious. Methods : This type of experimental research with the design of post test only control group using 24 well culture T47D mammary cancer cells in RPMI 1640 medium, each well containing 2x105 / 200μl. Four well to treatment with the fraction of ethanolic extract of stem anthill at a concentration 0 μgr / ml; 31.25 μgr / ml; 62.5 μgr / ml and 125 μgr / ml. Assessment : The meaning of display p21, ki67 declared Score Cytology Results: Regression test of correlation showed a positive relationship between the increase in the concentration of sarang semut with the expression levels of p21 R2 = 0.889 Extract sarang semut showed a negative relationship between the increase in the concentration of sarang semut with a decrease on the expression of ki67 R2 = 0.827 Conclusion : The fraction of ethanolic extract sarang semut decrease the level of ki67 expressions and increase the level of p21 expressions Keyword : sarang semut (Myrmecpodia pendens, Merr & Perry), p21 and ki67