PERBEDAAN DERAJAT INFEKSI DAN HITUNG KUMAN ANTARA PENGGUNAAN NYLON DARN, MESH MAKROPORI MONOFILAMEN DAN MULTIFILAMEN PADA OPERASI BERSIH TERKONTAMINASI Penelitian Eksperimental Pada Tikus Wistar

Abstract

Background: Hernia repair in clean-contaminated or contaminated operations, as in incarcerated hernia operations, are still debated. Nylon darn repair technique, which foreign bodies are less than mesh, is cheap and effective for hernia repair despite the fact of its unpopularity. This research is aimed to analyze difference of infection occurence and bacterial count, and its correlation, between nylon darn, macroporous monofilament and multifilament mesh in clean-contaminated operations, in a rat model. Methods: A randomized, bias-free laboratory experimental study was conducted on thirty wister rats. Sample was divided into three groups and applied nylon darn or macroporous monofilament or multifilament mesh. Infection occurence was noted based on Hulton’s criteria, while bacterial count using Spread-Plate method. Results were assessed using Kruskal-Wallis, Mann Whitney, and Spearman tests as appropriate. Results: There was a significant difference in bacterial count (p=0.006), while no difference in infection occurence of all groups (p=0.13). There were significant differences between macroporous multifilament mesh vs nylon darn (p=0.005) and macroporous monofilament mesh (p=0.007). There was no difference in bacterial count between nylon darn vs macroporous monofilament mesh (p=0.940). There was strong correlation between infection occurence and bacterial count (p=0.000; Spearman’s rho 0.662). Conclusion: Bacterial count in the application of macroporous multifilament mesh was significantly higher than nylon darn and macropore monofilament mesh, while there was no difference between nylon darn and macroporous monofilament mesh. There was no difference in infection occurence of all groups despite its strong correlation with bacterial count. Perhaps, it will be possible to note a significant difference of the infection occurence in clinical observation since surgical site infection occurs within 1 year after implant is in place. Key words : nylon darn, mesh, clean-contaminated operation, infection