UJI TOKSISITAS AKUT BIOPIGMEN KAROTENOID SIMBION BAKTERI DENGAN INVERTEBRATA LAUT (KAJIAN TERHADAP DUODENUM MENCIT BALB/C)

Abstract

Background: Biopigmen Carotenoid is the example of bioactive compounds that can be found in the sea marine microorganisms such as sea bacteria. One of the chemical compounds is tannin. High dose of tannin might cause excessive astringent effect leading to irritation on intestinal mucous membrane. The objective of this experiment was to know the effect of graded dose of biopigmen karotenoid in an acute manner on histopathological appearance of Balb/c mice duodenum. Method: This research was an experimental study using The Post Test Only Controlled Group Design. 25 male Balb/c mice were divided into 1 control group and 4 treatment groups. The treatment was given only on the first day. The control group was given aquadest. The other groups, P1 was given biopigmen carotenoid with 5 mg/kgBB dose, P2 50 mg/kgBB, P3 500 mg/kgBB, and P4 2000 mg/kgBB. At 8th day, the Balb/c mice were terminated, duodenum were made into slides. Result: Oneway Anova test that was used to observe duodenum histopathological showed significant difference between groups with p=0,001. There were significant difference between K-P3 (p=0,033), K-P4 (p=0,001), P1-P4 (p=0,002) and P2-P4 (p=0,016). Conclusion: The study shown that biopigmen carotenoid give a significant difference of duodenal histopathological appearance with control group, as erosion and ulceration.