UJI TOKSISITAS AKUT BIOPIGMEN KAROTENOID SIMBION BAKTERI DENGAN INVERTEBRATA LAUT (KAJIAN TERHADAP GINJAL MENCIT BALB/C)

Abstract

Background: Indonesia has a huge marine resources. Caroteoid pigment found in many marine organisms is a provitamin A compound that plays an important role in the physiologic function of the body. Researcher want to know whether any acute effect of biopigment carotenoid symbionts marine invertebrate (Jorunna funebris) with bacteria (Virgibacillus salaries) as one alternative source of carotenoid biopigmen. Method: This experimental Post Test-Controlled Only Group Design study was using 25 male Balb/c mices that were divided into five groups, one control group (K) that had been given standard food, and four treatment group (P1, P2, P3, P4) that had been given biopigment carotenoid symbionts marine invertebrate with bacteria treatment in 5 mg/kg BW, 50 mg/kg BW, 500 mg/kg BW, and 2000 mg/kg BW dosages via gastric sonde once, and being observed for a week. On 8th day, those mices were terminated to be observed the macroscopic appearance and the histopatological appearance. Microscopic data was analyzed by Oneway-Anova test and continued by Post-Hoc test. Result: There was no significant difference in kidney’s macroscopic appearance among groups, whereas there were significant differences in kidney’s microscopic appearance, there were: K-P2, K-P3, K-P4, P1-P3, P1-P4, P2-P4 (p=0.001), P2-P3 (p=0.029), and P3-P4 (p=0.002). Conclusion: Acute treatment of biopigment carotenoid has not make significant difference on the macroscopic appearance of kidney between control and treatment group and within each treatment group, but the treatment makes differences on the microscopic appearance of kidney between control and treatment group and within each treatment group.