Effects of Magnetic Pole on Orientation of Bull Sperm Treated by Dithiothreitol or Heparin

Pham, Du Ngoc and Shinjo, Akihisa and Kurnianto, Edy (2001) Effects of Magnetic Pole on Orientation of Bull Sperm Treated by Dithiothreitol or Heparin. Asian-Australasian Journal of Animal Sciences, 14 (2). pp. 155-162. ISSN 1011-2367

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Abstract

Effect of the north (N) or south pole (S) of a magnet and dithiothreitol (DTT) or heparin sodium (H) on the orientation of bull sperm were studied. Sperm were collected from four Japanese Black bulls of Okinawa Prefectural Livestock Experimental Station were treated with various concentrations of DTT or H during the first and sixth days and were then exposed them to N or S with a magnetic field of 3,000 Gauss for 24 hours. Experimental results showed that both N and S significantly increased the perpendicular oriented percentage of bull sperm in most treatments not treated or treated with DTT or H. Anisotropy of magnetic field of bull sperm was the main cause which induced them to orient perpendicular to the magnetic direction. The results of the experiment also showed that, in most cases, the two polarities had the same effect on the orientation of bull sperm. However, in some cases, N exerted a stronger effect on the perpendicular percentage of bull sperm than S, such as with the 20 mM DTT treatment at the first and sixth days and the 100 units H treatment at the sixth day. These cases showed that the perpendicular oriented percentage of bull sperm at N was significantly higher than that of S. It was only the 100 units heparin treatment at S both at the first and sixth days that upward perpendicular orientation was higher than that at N. This might be due to the fact that protamin-DNA structure of a small number of bull sperm nuclei slightly changed because of heparin action. Keywords : Magnetic Pole, Bull Sperm, Orientation, Dithiothreitol, Heparin

Item Type:Article
Subjects:S Agriculture > SF Animal culture
Divisions:Faculty of Animal and Agricultural Sciences > Department of Animal Agriculture
ID Code:33372
Deposited By:Mr. Magister Ilmu Ternak Admin
Deposited On:13 Feb 2012 08:51
Last Modified:13 Feb 2012 08:51

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