EFEK JUS TOMAT TERHADAP KADAR BILIRUBIN TOTAL TIKUS WISTAR YANG LEUKOSITOSIS SETELAH DIBERI PAPARAN ASAP ROKOK

Abstract

Background: Components of gas and tar in cigarette smoke contain many free radicals which can cause overconsumption of bilirubin level to overcome oxidative stress caused by those free radicals. Tomato has antioxidant elements can counteract free radicals such as vitamin C, vitamin E, and especially lycopen that has strength in capturing singlet oxygen two times more than the ability of beta caroten. Based on that experience, this study aims to prove antioxidant effect of tomato juice to see elevated level of bilirubin. Methods: This study is an experimental research laboratory with the approach of The Post Test Only Control Group Design. The study population was wistar rats that developed in the laboratory of the Universitas Negeri Semarang, is selected by simple random sample is divided into two groups where each group contained five rats. In this first study as a control group given only one stick of cigarette smoked per day and treatment group were given cigarette smoked and tomato juice 19 ml/day. Treatment given for 30 days, at day 31 performed blood sampling. Then calculated the total bilirubin level. Result: Result of research showed rate mean of total bilirubin level at group of control 0,19 ± 0,02 mg/dl and total bilirubin level at group of treatment 0,22 ± 0,03 mg/dl. Parametric test results of independent T-test found no significant difference between control and treatment groups in the total bilirubin level (p=0,123). Conclusion: There are no significant difference in the total bilirubin level in wistar rats exposed to cigarette smoke are given tomato juice with those not given tomato juice.