PENGARUH PEMBERIAN EKSTRAK DAUN PEPAYA (Carica Papaya L) 100% TERHADAP BAKTERI Staphylococcus aureus DARI PIODERMA

Abstract

Background: Pioderma is a skin disease marked by pus formation by pyogenic bacteria. The main caused of pioderma is Staphylococcus aureus, and Streptococcus β Hemolitikus. According to some researches, papaya leaf has antibakterial effect on positive and negative bacteria. Objective: The purpose of this research were to know the Minimum Inhibitory Concentration (MIC) of papaya leaf extract (Carica papaya L) and to know the effect of papaya leaf extract (Carica Papaya L) on Staphylococcus aureus bacteria from pioderma caused by Staphylococcus aureus Method: Preeliminary test is done to determine Minimum Inhibitory Concentration (MIC) of papaya leaf extract. Then pus from pioderma patients are swabbed, followed by gram staining. If Staphylococcus aureus is found, pus is cultured on nutrient agar, then be incubated at 37˚C for 24 hours. Then bacteria suspension are made from the agar and cultured on both agar previously added with papaya leaf extract and plain agar without papaya leaf extract as a positive control. Result: From 30 samples of positive Staphylococcus aureus, 1 sample was not found Staphylococcus aureus, while the 29 were found Staphylococcus aureus. Fischer test showed insignificant with p=1 Conclusion: There is no effect of papaya leaf extract (Carica Papaya L) 100% on Staphylococcus aureus bacteria from pioderma caused by Staphylococcus aureus.