Perbandingan Ekstrak Daun Kemangi (Ocimum basilicum L.)dengan Ketokonazol 2% dalam Menghambat Pertumbuhan Candida sp. pada Kandidiasis Vulvovaginalis

Abstract

Background: Tendency of people to return to the natural ingredients begin to increase. Basil is one of the many traditional medicinal plants which use by communities as medicinal plants and culinary herbs.Vulvovaginalis candidiasis is a genital infection in women, cause a lot of complaints and discomfort, ketoconazole is standard antifungal that often used in the treatment of this infection. This study aims to compare the basil leaf extract with 2% ketoconazole in inhibiting in vitro growth of Candida sp. on Vulvovaginalis candidiasis. Methods : This study is an experimental research laboratory with post test only control group design. The sample consisted of 30 cultures of Candida sp.(+) on Sabouraud Dextrose Agar (SDA) medium from patients of vaginal candidiasis based on clinical finding and gram staining. The colonies of Candida sp. were diluted in o,9% NaCl to make the solution equal to 0,5 Mc Farland, then 0,1 cc of it was taken and cultivated into SDA medium containing extracts of basil leaves 50% and SDA containing ketoconazole 2%. The media were incubated at 37oC for 24-48 hours. Data obtained by looking at the growth of colonies of candida sp. on both types of media. Data analysis using the chi-square with degrees of significance p ≤ 0,05. Result : on 30 SDA medium containing extracts of basil leaves 50%, there are 2 tubes of Candida sp.(+), whereas in 30 tubes os 2% ketoconazole medium contained 8 tubes of candida sp.(+). The result of chi-square test was significant p = 0,038. Conclusion : Basil leaves extracts (Ocimum basilicum L) inhibited in vitro growth of Candida sp. better than 2% ketoconazole in vulvovaginalis candidiasis.