Analysis content of lycopen on Tomato Using Spectrophotometer

Abstract

Tomato (Lycopersicum esculentum) is one of the potential agricultural products, healthy, have a promising market prospect and has a nutrient composition is fairly complete, especially lycopene. Lycopene is an antioxidant yangt very strong. His ability to control the singlet oxygen (oxygen free radicals in the form) 100 times more efficient than vitamin E or 12 500 times that of the gluthation In this lab using Optima Spectrophotometer SP 300 based on the phenomenon of absorption of light by certain chemical species in the region of visible light (visible) . Solution of tomato fruit and tomato vegetables coupled with ethanol solvent with a ratio of 1:1 and 1:2 by heating 30 ° C and 40 ° C and obtain results for large levels of lycopene in tomato fruit is shown at 30 ° C with 0.73 absorbance and the concentration of 3.5. Solvent tomatoes with a temperature of 30 ° C have the greatest levels of lycopene, it can be concluded that the smaller the transmittance of the solution, the greater the absorbance, or vice versa, both have an inversely proportional relationship. Based on experiments on measuring the absorbance obtained linear regression, y = 0.01 x - 0.003 with a value of R2 = 0.999. And y = 0.000 x + 0.015 with R2 = 0.995. From the experimental results of the analysis levels of lycopene in tomatoes each 4 times Keywords : Spectrophotometer, Tomato analysis, Lycopene