Identifikasi senyawa Flavonoid Fraksi Metanol Daei kulit batang

Abstract

Identifikasi dan eksplorasi senyawa kimia bahan alam yang terkandung dalam tumbuhan perlu dilakukan untuk pemanfaatan dan pendataan potensi sumber days alam hayati. Senya.wa kimia bahan alam golongan flavonoid yang terkandung dalam kulit batang Artocarpus COMMUni S Forst ( Kluweh ) dapat diidentifikasi pada elcstralc metanol. Serbuk kulit batang Artocarpus convnunis Forst ( Kluweh ) dieksiraksi menggunakan metanol dengan metode perkolasi. Pemisahan dilakukan dengan metode kromatografi dengan pelarut kloroform dan fasa diem silika gel. Setelah pemurnian, diperoleh senyawa X yang berwarna kuning muds Senyawa X yang diperoleh pada uji golongan fenolik dengan menambahkan FeCl3 1% berwarna merah yang berarti positif Identifikasi senyawa X dengan UV diperoleh panjang gelombang 313 nm ( pita I) dan 390 mu (pits II ), sedangkan identifikasi spelctra IR dapat diketahui senyawa X mempunyai gugus fungi OH ( v 4343 cm-1 ), C=0 ( v 1728 cm-1 ) dan C=C aromatik ( v 1651 cm-1 dan v 1593 em-1 ). Dari data yang diperoleh dapat disimpulkan senyawa X berasal dan golongan flavonoid, struktur molekulnya belum diketahui. Identification and exploration of natural chemical compound on plants are necessary for its utility and collecting data The objective of this research is to identify a kind of flavonoid compound on stem bark of kluweh (Artocarpus communis Forst.). The compound was extracted by metanol through percolation method. Chromatography method using chloroform as mobile phase and silica gel as stationary phase were applicated to separate the compound. Yellowish solid material was gained after purification. Then, that solid material would be tested by phenolic test with adding FeCl3 1%. Phenolic test showed that solid material had positive reaction that to be indicated of red colour. Analysis by spectroscopy UV showed that solid material had two wave length namely 313 run ( as peak I) and 390 nm ( as peak Another analysis, spectroscopy IR, also showed that solid material had three groups, OH group (4343 cm-I), C=0 group (1728 cm-I) and C aromatic group (1651 cm4 and 1593 cm I) respectively. From many analysis as mentioned above, that solid material is flavonoid compound with unknown molecule structure as conclusion.