KADAR TNF-aIL-6 DAN APOPTOSIS TROFOBLAST PLASENTA Pada Preeklampsia-Eklampsia dan non Preklampsia-Eklampsia

Abstract

Objectives: The aim of this study is to investigate the relation among TNF-a -6 concentration/expression with placental tissue infarction and apoptosis between PE-E and non PE-E. Material and method: Research subjects were 35 subjects, composed of 17 non PE-E and 18 PE-E parturient that delivery or hospitalized on Department of OBGYN Dr. Kariadi General Hospital Semarang. The concentration/expression of TNF- -6 were measured from vein blood sample and placental tissue by ELISA and immunohystochemistry (IHC) method. Apoptosis area was measured by IHC with Acridine orange staining. Results: The average of infarction on normal subjects was 12.5%, however, on PE-E was 35.3% (p=0.001). The average of apoptosis on normal subjects was 32.3 %, however, on PE-E subjects were 71.0% (p=0.001). The average of placenta TNFpg/ mL, on PE-E was 2.0 pg/mL (p<0.001). The average of serum TNFnormal subjects was 2.3 pg/mL, on PE-E was 2.8 pg/mL (p<0.001). The average of placenta IL- 6 concentration of normal subjects was 0.6 pg/mL, on PE-E was 1.3 pg/mL (p<0.001). The average of serum IL-6 concentration of normal subjects was 1.4 pg/mL, on PE-E was 2.0 pg/mL (p<0.001). There were a significant correlation among TNF-a -6 serum and placenta with apoptosis of placenta. Conclusion: Proinflammatory cytokines concentration on serum and placenta extract of subjects with PE-E were significantly higher than non PE-E. There was a significant correlation between proinflammatory cytokines concentration on serum or placenta with apoptosis of placental tissue. Keywords: TNF-a , IL-6, infarct, apoptosis, placenta, preeclampsia, eclampsia.