Efek Paparan Arus Listrik terhadap Jumlah Titik Hiperkontraksi Otot Jantung dan Kadar Kreatin Kinase - MB Serum Tikus Wistar

Abstract

Background: Electrocution can cause damages in tissue and body biochemical changes including heart muscles hypercontraction and elevation of creatine kinase–MB serum level. The purpose of this study is to prove effects of electrical current directly and through medium of water toward the amount of heart muscle fibre hypercontraction points and elevation of creatine kinase–MB serum level. Method: 54 wistar mice was chosen using simple random sampling. Samples then divided into 9 groups, i.e. group 1 – 4 had direct exposure to electrical current, 1-30 mA, 31-60 mA, 61-90 mA, 91-120 mA, respectively. Group 5 – 8 were exposed to electrical current through medium of water, 1-30 mA, 31-60 mA, 61-90 mA, 91-120 mA, respectively. Group 9 were not exposed to electrical current. After 7 days of adaptation, exposure of electrical current were performed then creatine kinase–MB were measured from sinus retroorbitalis using COBAS Integral 400 Plus-Roche and number of heart mucle fibre hypercontraction points were counted. Data analysis were performed using Spearman , Pearson correlation test and independent t test Result: Correlation value between the strength of exposure of electrical current and through medium of water with the amount of heart muscle fibre hypercontraction points were 0.981 (p = 0.000) and 0.423 (p = 0.020), while correlation number between the strength of exposure of electrical current and through medium of water with elevation of creatine kinase–MB serum level in wistar mice 0.256 (p = 0.172) and 0.337 (p = 0.068). Conclusion: There is a strong positive correlation between the strength of electrical current exposures with the amount of heart muscle hypercontraction points. There is no correlation between the strentgh of electrical current exposures with elevation of creatine kinase–MB level in wistar mice. There is a significant difference between the means of heart muscles hypercontraction points, but there is no significant creatin kinase-MB levels difference between direct electrical current exposure and the use of water as the medium. Keywords: electrical current, direct exposure, medium of water, heart muscle, hypercontraction, creatine kinase–MB