Viabilitas Bacillus sp. DUCC-BR-K1.3 dan Azotobacter chroococcum pada Bahan Pembawa Campuran Gambut dan Akar Pakis (Cycas rumphii Miq) Selama Penyimpanan 60 Hari

Abstract

Price of chemical fertilizers has increased to make the farmers to find alternative fertilizer. Alternative fertilizer is often identified with biofertilizers, biological product that is active in the form of inoculants consisting of one or more microbial strains that works to increase the efficiency of fertilization and soil fertility. The purpose of this study was to determine the viability of inoculant Bacillus sp. DUCC-BR-K1.3 and Azotobacter chroococcum on the carrier material mixture of peat moss and fern roots, and to know formula influence for long storage of inoculum and may increase the viability of both rhizobacteria. Bacillus sp. DUCC-BR-K1.3 has been tested to synthesize the hormone IAA (indole acetic acid), whereas A. chroococcum function as a biological agent N fixation. This study used the formulas of microbial inoculum is added to the sterile carrier material, namely a mixture of peat moss and fern root with the composition ratio of 1:1. Peat moss and fern root has been tested as carrier materials that are good for both rhizobacteria. The experimental design of this study was completely randomized design factorial. The first factor F1 formula inoculum (75% Bacillus sp. DUCC-BR-K1.3 & 25% of A. chroococcum), F2 (50% Bacillus sp. DUCC-BR-K1.3 & 50% of A. chroococcum), and F3 (25% Bacillus sp. DUCC-BR-K1.3 & 75% of A. chroococcum), while the second factor of the old store (T0: 0 days, T1: 15 days, T2: 30 days, T3: 45hari, and T4: 60 days). The results showed that among the three inoculum formula there is no real difference. 15 days old store is the old store that can improve the viability of Bacillus sp. DUCC-BR-K1.3 & A. chroococcum.