The Effect of Superovulation on Corpus Luteum in Simbrah Cow

Abstract

Twenty Simbrah cows (body weight ranging from 404 – 496 kg, and age ranging from 3 to 5.5 years) were used to study the effect of superovulation on corpus luteum. Trial cows were synchronized by using PGF2a with multi level method and they were superovulated by 5 treatments and 4 replications. The treatments were P1 = superovulated by 40 mg of FSH of intramusculary injected and decreased level, P2 = superovulated by 10% of FSH of intraovary injected and decreased level, P3 = superovulated by 5% of FSH of intraovary injected and decreased level, P4 = superovulated by 300 IU (10%) of PMSG of intraovary injected and P5 = superovulated by 150 IU (5%) of PMSG of intraovary injected. Amount of CL, follicle was calculated by rectal palpation on day-7 after artificial insemination. Result of this study showed that superovulation on Simbrah cow resulted 97 of corpus luteum with average of 4.85 ± 3.1. The best treatment was superovulation of P1 with corpus luteum average of 9.0 ± 4.68 (p<0.05). Superovulation of P2, P3, P4 and P5 resulted similarity in number of corpus luteum (P>0.05). The number of follicle was 30 with the average of 1.5 ± 1.7. This study indicated that the best superovulation was the treatment of P1 in which resulted more corpus luteum. Keywords : Superovulation, corpus luteum, Simbrah cow