Blood Protein Variability as Biogenetic Parameter of Java Cattle

Abstract

Java cattle is the potential cattle to be developed as the superior cattle in Indonesia. It is possible that Java cattle have specific gene character that is not found in other kind of cattle in Indonesia. The objective of this study was to obtain data of the gene tipology through the analysis of blood protein variability by electrophoretic method. Blood used as the sample originated from 30 heads of Java cattle at Brebes Regency, Central Java, in which those were taken purposively. Variables observed were blood protein variability, that were albumin (Alb), transferrin (Tf), ceruloplasmin (Cp) and post transferrin (Ptf). Banding protein obtained was used for calculating the values of gene frequency and average of the heterozygosity.The results showed that: (1) At albumin (Alb) locus, gene frequency of allele B, A and C was 0.634; 0.183; and 0.183, respectively; (2) At transferrin (Tf) locus, gene frequency of allele D3, D1 and D2 was 0.533; 0.15; and 0.317, respectively; (3) At ceruloplasmin (Cp) locus, gene frequency of allele F and S was 0.563 and 0.467; (4) At post transferrin (Ptf) locus, gene frequency of allele S and F was 0.8 and 0.2. The average of heterozygosity was 0.199. Keywords : Java cattle, blood protein, heterozygosity, gene frequency