KADAR SENG PLASMA DAN INTERLEUKIN-12 PADA ANAK USIA 6-14 TAHUN DI DAERAH ENDEMIS MALARIA PLASMA ZINC LEVELS AND INTERLEUKIN-12 IN CHILDREN 6-14 YEARS OLD IN MALARIA ENDEMIC AREAS

Abstract

ABSTRACT Background. Malaria is one of the main health problems in children. The interaction between immunity and malaria is complex, involving mostly immunity system. Zinc is a micronutrien that plays esential role in many aspect of human metabolism. Study of interaction between zinc and Interleukin-12 in malaria endemic areas was limited. Aims. To determine the correlation between zinc plasma levels and Interleukin-12 (IL-12) in 6-14 years old children in malaria endemic areas Methods. A cross sectional study was done on 103 children 6-14 years old in Madrasah Ibtidaiyah (MI) Kalitapas and MI Bleber village, Purworejo district from March until April 2009. Plasma zinc levels was measured by Atomic Absorption Spectrophotometer, Interleukin-12 was measured by ELISA, thin and or thick blood film and Rapid Diagnostic Test was used for determine malaria infection status. Statistical analysis was done using Independent T-test, Mann Whitney, Pearson, and Spearman. Result. Subject: 103 children 6-14 years old. Boys predominate, with mean age 9,7±1,9 years. Mean plasma zinc levels was 146,38 ± 41,14 µg/dL. Mean IL-12 concentration was 3,3 ± 3,48 pg/ml. Negative malaria status predominate (98,1%). Correlation test between zinc plasma levels and IL-12 concentration was positive (r=+0,20, p=0,04). Conclusion. There is a significant weak correlation between zinc plasma levels with IL-12 concentration. Keywords. Zinc plasma levels, Interleukin-12, malaria. ABSTRAK Latar Belakang. Malaria masih merupakan masalah kesehatan pada anak. Imunitas terhadap malaria sangatlah kompleks, melibatkan hampir semua sistem imunitas tubuh. Seng berperan penting dalam sistem imunitas tubuh. Interaksi antara seng dan Interleukin-12 di daerah endemis malaria masih terbatas. Tujuan. Mengetahui hubungan kadar seng plasma dan Interleukin-12 (IL-12) pada anak usia 6-14 tahun di daerah endemis malaria Metode. Penelitian belah lintang pada 103 anak usia 6-14 tahun di Madrasah Ibtidaiyah (MI) Desa Kalitapas dan Desa Bleber Kabupaten Purworejo, dari Maret sampai April 2009. Kadar seng plasma diperiksa dengan metode Atomic Absorption Spectrophotometry, kadar IL-12 dengan metode ELISA, sedangkan status infeksi malaria berdasarkan pemeriksaan darah tebal dan atau tipis serta Rapid Diagnostic Test. Analisis statistik menggunakan Independent T-test, Mann Whitney, Pearson, dan Spearman. Hasil. Dari 103 anak usia 6-14 tahun, sebagian besar berjenis kelamin laki-laki dengan rerata umur 9,7 ± 1,93 tahun. Rerata kadar seng plasma 146,4 ± 41,14 µg/dL. Rerata kadar IL-12 3,3 ± 3,48 pg/ml. Status infeksi malaria sebagian besar negatif (98,1%). Uji korelasi antara kadar seng plasma dengan IL-12 r=+0,20, p=0,04. Simpulan. Terdapat korelasi bermakna derajat rendah antara kadar seng plasma dengan IL-12 pada anak usia 6-14 tahun di daerah endemis malaria. Kata kunci: Kadar seng plasma, Interleukin-12, malaria