PENGARUH PEMBERIAN Andrographis paniculata TERHADAP PRODUKSI INTERFERON GAMMA PADA MENCIT YANG DIINOKULASI Plasmodium berghei ANKA

Agustin, Yuanita (2008) PENGARUH PEMBERIAN Andrographis paniculata TERHADAP PRODUKSI INTERFERON GAMMA PADA MENCIT YANG DIINOKULASI Plasmodium berghei ANKA. Undergraduate thesis, Faculty of Medicine.

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Abstract

Background: Andrographis paniculata (A. paniculata) can stimulate immune response of BALB/c mice during the infection of Plasmodium berghei ANKA (P. berghei ANKA). While interferon-gamma (IFN- γ) has been proved to be protective during the infection. Yet there is no proof that the protective mechanism of the herb is mediated by IFN- γ. The objective of this study was to find out whether A. paniculata has an effect on IFN- γ producing capacity in mice inoculated by P. berghei ANKA. Methods: This study was pure experimental with post test only control group design. It used 36 BALB/c mice, divided randomly into treated and control groups. Each mice in treated group received 16,608 mg methanol extract of A. paniculata, solved in CMC-Na 0,5%, started from 7 days pre-inoculation until 8 days post-inoculation (p.i.). While control group received CMC-Na 0,5% 0,1 ml/day/mice with same duration. All of the mice was inoculated with P. berghei ANKA on day 7 after the treatment started. IFN- γ producing capacity was measured on day 3, 6, 8 p.i. Data was processed using Kruskal Wallis and Mann Whitney statistical analysis test. Results: IFN- γ producing capacity of treated group was significantly higher than control group on day 3 p.i. of P. berghei ANKA. In treated group, there was significant difference of IFN- γ concentration between day 3 and 6 p.i. In control group, there were significant differences of IFN- γ concentration between day 3 and 6, and also day 3 and 8 p.i. of P. berghei ANKA. Conclusion: A. paniculata herb extract increase spleenocytes’ IFN- γ producing capacity on day 3 p.i. of P. berghei ANKA. Keywords: Andrographis paniculata, IFN-γ, Plasmodium berghei ANKA

Item Type:Thesis (Undergraduate)
Subjects:R Medicine > R Medicine (General)
Divisions:Faculty of Medicine > Department of Medicine
Faculty of Medicine > Department of Medicine
ID Code:24543
Deposited By:Ms Lutfiatun Heni
Deposited On:10 Dec 2010 11:12
Last Modified:10 Dec 2010 11:12

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