PENGARUH Andrographis paniculata TERHADAP KAPASITAS PRODUKSI INTERLEUKIN 12 PADA MENCIT YANG DIINOKULASI Plasmodium berghei ANKA

Nurohmah, Siska (2008) PENGARUH Andrographis paniculata TERHADAP KAPASITAS PRODUKSI INTERLEUKIN 12 PADA MENCIT YANG DIINOKULASI Plasmodium berghei ANKA. Undergraduate thesis, Faculty of Medicine.

[img]
Preview
PDF - Published Version
176Kb

Abstract

Background: Malaria is a disease caused by intracellular parasite. Interleukin-12 (IL-12) acts as an important cytokine in cellular immune response required for eliminating parasite. Previous studies showed that Andrographis paniculata (A. paniculata) has a protective role during malaria infection, but there is no evidence that the protective mechanism of the herb is mediated by IL-12. Therefore, the objective of this study is to find out whether A. paniculata has an effect on IL-12 producing capacity in mice inoculated with Plasmodium berghei (P. berghei) ANKA. Methods: This study was true experimental research using post test only control group design. Total sample used were 36 BALB/c mice, divided equally into treated and control groups. Treated groups were received 16.608 mg A. paniculata herb extract diluted in 0.1 ml CMC Na administered orally per day, started from 7 days pre-inoculation of P. berghei until 8 days post-inoculation (p.i.). Control group received 0.1 ml CMC-Na 0.5% in the same duration. IL-12 producing capacity was measured on day 3, 6, and 8 p.i. Data were analyzed by Kruskall-Wallis and Mann-Whitney test. Results: No significant difference in IL-12 producing capacity between treated and control group (day 3 p=0.671; day 6 p=1.000; day 8 p=0.971). The same result went for IL-12 producing capacity among days 3, 6, and 8 p.i. in both treated and control groups (p=0.872). Conclusion: A. paniculata does not significantly affect IL-12 producing capacity in BALB/c mice inoculated with P. berghei ANKA. Keywords: Interleukin-12, Plasmodium berghei ANKA, Andrographis paniculata.

Item Type:Thesis (Undergraduate)
Subjects:R Medicine > R Medicine (General)
Divisions:Faculty of Medicine > Department of Medicine
Faculty of Medicine > Department of Medicine
ID Code:24541
Deposited By:Ms Lutfiatun Heni
Deposited On:10 Dec 2010 10:27
Last Modified:10 Dec 2010 10:27

Repository Staff Only: item control page