UJI BANDING EFEKTIVITAS MERANG (Rice straw) 50 % DENGAN KETOKONAZOLE 1% SECARA IN VITRO TERHADAP PERTUMBUHAN Pityrosporum ovale PADA KETOMBE

Abstract

Background: Merang (Rice straw) is the stem of the rice plant (Oryza sativa) that used as a traditional drug in the treatment of dandruff. Ketokonazole 1% is an antifungal synthetic azole drug which has a broad spectrum dan a high effectivity to inhibit ergosterol synthesize that is important to keep the integrity of the fungal membrane. Dandruff is a superficial fungal infection with itchy scaling scalp as a common symptom. Pityrosporum ovale is the causing agent of dandruff. Objective: To compare the effectiveness of 50% Rice straw’s ash water versus 1% ketokonazole in vitro to the growth of Pityrosporum ovale in dandruff. Method: This research was done by using an experimental study. As samples were 30 patients of dandruff with clinical founding. Diagnosis of Pityrosporum ovale was based on the result of microscopic examination of skin scraping from samples, with using KOH ,and inoculation on the SDA olive oil added with Chloramphenicol 50µg/cc medium at 370 C for 3 days. The colonies of Pityrosporum ovale were diluted in sterile 0,9% NaCl until its turbidity equal to 0,5 Mc Farland standard. As many as 0,1 cc of solution was cultivated on the olive oil SDA media supplemented with 50% Rice straw’s ash water and 1% ketokonazole and then the media were incubated at 370 C for 3 days. The difference of growth proportion was analyzed by Chi-Square Test with the degree of significance of p<0,05. Result: 30 media of olive oil SDA which contained 50% Rice straw’s ash water 29 medias(48,33%) were found (+)/ positive for Pityrosporum ovale growth and 1 media (1,67%) were found (-)/ absence for Pityrosporum ovale growth. Meanwhile, 30 media which contained 1% ketokonazole, 5medias (8,33%) were found positive and 25medias (41,67%) were found negatives. The result of the Chi-Square Test is significant with p=0,000. Conclusion: There is a significant difference between the effectiveness of 50% Rice straw’s ash water with 1% ketokonazole in inhibiting the growth of Pityrosporum ovale in dandruff. The 50% Rice straw’s ash water can not replace 1% ketokonazole in inhibiting the growth of Pityrosporum ovale in dandruff. Key words : Dandruff, Pityrosporum ovale, 50% Rice straw’s ash water, 1% Ketokonazole