PERBANDINGAN EFEKTIVITAS EKSTRAK NANAS 100% DENGAN KETOKONAZOL 2% SECARA IN VITRO TERHADAP PERTUMBUHAN Pityrosporum ovale PADA KETOMBE

Abstract

Background: Pineapple extract is a traditional herbal medicine which have many benefits e.g antiinflammation, antioxidan, hydrolysing protein and skin debridement. Ancient people used to take pineapple for dandruff treatment. Ketoconazole is an effective antifungal agent for treating dandruff that works by blocking ergosterol synthesis that is an important component for membrane integrity of fungal cell. Dandruff is the excessive scales production of the scalp. Pityrosporum ovale seems to have an important role in provoking dandruff. Objective: To compare the effectiveness of 100% pineapple extract with 2% ketoconazole in vitro to the growth of Pityrosporum ovale taken from dandruff. Method: This study was done by an experimental design. As samples were 30 patients of dandruff with clinical founding and for the last 3 days, are not having shampoo, with positive result of Pityrosporum ovale culture. Diagnosis of Pityrosporum ovale was based on the result of microscopic examination of samples using KOH added blue black parker ink, and inoculation on the SDA olive oil added Amoxycillin 10μg/cc medium at 370 C for 3 days.The colonies of Pityrosporum ovale were diluted in steril 0,9% NaCl to make the solution equal to 0,5 Mc Farland standard. As many as 0,1 cc of solution was cultivated on the olive oil SDA media supplemented with 100% pineapple (Ananas comosus) extract with 2% ketoconazole, and then the media were incubated at 370 C for 3 days.The difference proportion of growth was analyzed by chi square test with degree of signifance of p<0,05. Result: 30 media of olive oil SDA which contained 100% pineapple (Ananas comosus) extract, 27 (90%) were found (+)/positive Pityrosporum ovale growth and 3(10%) were found (-) /absence growth. And 30 media which contained 2% ketoconazole, 3(10%) were found positive and 27(10%) were found negative. The result of chi square test is significant p=0,000 (p<0,05). Conclusion: There was a significant difference between the effectiveness of 100% pineapple (Ananas comosus) extract with 2% ketokonazole in inhibiting the growth of Pityrosporum ovale from dandruff. Accordingly, pineapple extract can't replacing ketoconazol 2 % for inhibiting Pityrosporum ovale in treating dandruff Key words : Pityrosporum ovale, 100% pineapple(Ananas comosus) extract, 2% Ketoconazole