PENGARUH LAMA INKUBASI TERHADAP PERTUMBUHAN KUMAN PADA KULTUR DARAH PENDERITA SEPTIKEMIA DI RSUP DR. KARIADI SEMARANG PERIODE JANUARI - APRIL 2008

Abstract

Backgrounds: Septicemia is a dangerous systemic bacterial infection which has a high mortality rate. Immediate diagnosis empirical therapy of antibiotic plays important role on the patient prognosis. Incubation period is one of many factors that influence blood culture result which is a routine procedure to diagnose septicemia. Positive results were found far from expectation. Therefore, study on influence of incubation period was needed for increase blood culture positive rate. Methods: This was an analytic observational study cross-sectional design sample consist of 270 blood culture bottles which were examined from surgery ward, intern ward, and ICU of Dr. Kariadi Hospital on January-April 2008. Blood sample was taken aseptically, cultured and incubated in ten days at BACTEC 9050. Bacterial growth, incubation period, bacterial species and antibiotic using history were observed. Data was processed with McNemar test using SPSS 15.0 for windows. Results: There was no significant result in this study. The result showed that 97,5% of positive results were found within the first three days. Average incubation periods were 2,09 days. Conclusion: Ten days incubation did not increase blood culture positive rate from five days incubation. Keywords: septicemia, blood culture, incubation period, bacterial growth.