PERBANDINGAN TINGKAT SENSITIVITAS DAN SPESIFISITAS PADA PEMERIKSAAN INFLUENZA A DENGAN MENGGUNAKAN RAPID TES DAN REAL TIME-REVERSE TRANSCRIPTASE PCR (rRT-PCR)

Abstract

Background : Influenza is a disease caused by influenza viral which made high morbidity rate and possibility of serious complication in infant and the elderly people. Instrument with good sensitivity and specificity is needed to make an early detection of illness. Objective : This research is aimed to compare the sensitivity and specificity between rapid test and real time-reverse transcriptase PCR. Method : This research is a diagnostic test. Total samples of 418 patients were obtained from data which started from November 2007-April 2008 at The Laboratory of Avian Influenza Medical Faculty Diponegoro University. Samples were taken from nasal swab and a questioner was filled based on the rapid test result of patients with Influenza Like-Illness came from Solo, Cirebon, Malang, Boyolali. After samples sent to laboratory then researchers did diagnostic test with PCR. Both of results tests would be compared using diagnostic test. Result : The sensitivity rate of rapid test include all data was 23,25%, the specificity was 99,39%, the positive predicted value was 90,9%, and the negative predictive value was 83,3%. The diagnostic test in children category gave result for sensitivity was 30,43%, the specificity 99,22%, the positive predicted value was 93,33%, and the negative predicted value was 79,87%. The diagnostic test in older category gave result for sensitivity was 18,75%, the specificity 99,52%, the positive predicted value was 85,71% and the negative predicted value was 89,03%. Conclusion : The sensitivity rate of rapid test was low but the specificity was high. Key word : sensitivity, specificity, rapid test, RT-PCR