PERBANDINGAN EFEKTIVITAS AIR PERASAN BAWANG MERAH (Allium ascalonicum L.) 100% DENGAN KETOKONAZOL 2% SECARA IN VITRO TERHADAP PERTUMBUHAN Pityrosporum ovale PADA KETOMBE

Abstract

Background: Dandruff is a scalp disorder that show a lot of small silvery white-grey scales, sometimes completely with pruritus, caused by Pityrosporum ovale. Ketokonazol is a topical antifungal medication that used as drugs of choice of dandruff treatment. Allium ascalonicum L. (shallot) is one of traditional herbal medicine that almost used to treat dandruff. This study was aimed to compare the effectiveness of 100% Allium ascalonicum L. with 2% ketoconazole in vitro to the growth of Pityrosporum ovale in dandruff. Method: This was an experimental study using post-test design only with 30 patients of dandruff with clinical founding and for the last 3 days, were not having shampoo. Diagnosis of Pityrosporum ovale was based on the result of microscopic examination of samples using KOH added by blue black parker ink, inoculated on the Sabouraud Dextrose Agar olive oil added by Amoxycillin 10μg/cc medium (370 C for 3 days). The colonies of Pityrosporum ovale were diluted in steril 0,9% NaCl as equal as 0,5 Mc Farland standard. As many as 0,1 cc of solution was cultivated on the olive oil Sabouraud Dextrose Agar media supplemented with 100% Allium ascalonicum L. and 2% ketoconazole incubated at 370 C for 3 days. The difference proportion of growth was analyzed by Chi Square test with degree of signifance of p<0,05. Result: 30 media of olive oil Sabouraud Dextrose Agar contained 100% Allium ascalonicum L., all media (30/100%) were (+)/positive for Pityrosporum ovale growth. Meanwhile, 30 media contained 2% ketoconazole, 3(10%) were found positive and 27(90%) were found negative. The Chi Square test is significantly different (p=0,000). Conclusion: There was significant difference between the effectiveness of 100% Allium ascalonicum L. with 2% ketokonazole inhibiting the growth of Pityrosporum ovale in dandruff. Shallot (Allium ascalonicum L.) couldn’t used to substitute ketokonazole as alternative therapy inhibiting growth of Pityrosporum ovale in dandruff. Key words : Dandruff, Pityrosporum ovale, Shallot (Allium ascalonicum L.), Ketoconazole