UJI BANDING EFEKTIVITAS AIR RENDAMAN KANGKUNG (Ipomea reptans) DENGAN KETOKONAZOL 1% SECARA IN VITRO TERHADAP PERTUMBUHAN Pityrosporum ovale PADA KETOMBE

Abstract

Background: Pityrosporum ovale, a lipophilic yeast of the genus Malasezzia that is a common comensal of the scalp, increases in dandruff. Ketoconazole is an antimycotic of imidazole group. The use of ketoconazole as an active ingredient in antidandruff is based on the role of Pityrosporum ovale as causal microorganism. Traditional treatment to eliminate dandruff can be found in the population too. The water-immersed kangkung can be used to eliminate dandruff. In this case the antimicrobial or antifungal effects of this plant are suspected as having important role. Objective: To find out the effectivity of water-immersed kangkung (Ipomea reptans) in comparison with 1% ketoconazole in vitro on the growth of Pityrosporum ovale in dandruff. Method: This research was done by using an experimental study. The samples were 30 patients of dandruff with clinical founding. The specimen for investigation are scrapped scales from the scalp of the patients. It was followed by culture in Sabaroud Dextrose Agar (SDA) olive oil at temperature of 370C for 3 days. The result of the culture (+) are diluted in sterile solution of 0.9% NaCl and its turbidity is made equal with 0.5 McFarland solution, then 0.1 cc was taken and cultivated in medium of SDA olive oil that contained water immersed kangkung and 1% ketoconazole. Then the medium was entered in an incubator at temperature of 370C for 3 days. The data were analyzed using chi square test with degree of significance of p<0,05. Result: 30 media of olive oil SDA that contained water-immersed kangkung, 30 (50%) were stated as (+) that revealed growth of Pityrosporum ovale and none (0%) was stated as (-) that revealed no growth of Pityrosporum ovale. Whereas from 30 media of SDA olive oil that contained 1% ketoconazole, 5 (8.3%) were stated as (+) that revealed growth of Pityrosporum ovale and 25 (41.7%) were stated as (-) that revealed no growth of Pityrosporum ovale. The chi square test is significant p=0,0000. Conclusion: Water-immersed kangkung is not effective compared with ketoconazole 1% in retarding the growth of Pityrosporum ovale in dandruff. Key words : Water-immersed kangkung, 1% ketoconazole, Pityrosporum ovale dandruff.